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thiW [2019-04-24 13:40:04]
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thiW [2019-04-24 13:40:04]

thiamine ECF transporter (ATP-binding protein)
Locus
BSU13220
Isoelectric point
5.71
Molecular weight
54.48 kDa
Protein length
547 aa Sequence Blast
Gene length
1644 bp Sequence Blast
Function
thiamine uptake
Product
thiamine ECF transporter (ATP-binding protein)
Essential
no
Synonyms
ykoD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → ECF transporter → Class I ECF transporter
  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,388,809 → 1,390,452

    The protein

    Protein family

  • ECF transporter family

    • Structure

  • 4HUQ (folate transporter from Lactobacillus brevis, 34% identity) PubMed

    • Localization

  • membrane associated (via ThiV-ThiX) PubMed

    • Expression and Regulation

    Operons

    Genes
    thiU-thiV-thiW-thiX
    Description
    PubMed

    Regulatory mechanism

  • Thi-box: RNA switch, via RNA switch, in Thi-box regulon

    • Regulation

  • repressed by thiamine (Thi-box) PubMed
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    Biological materials

    Mutant

  • MGNA-A755 (ykoD::erm), available at the NBRP B. subtilis, Japan
  • BKE13220 (ΔthiW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA, downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT
  • BKK13220 (ΔthiW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA, downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT

    • References

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